PDBe 3plf

X-ray diffraction
1.92Å resolution

Reverse Binding Mode of MetRD peptide complexed with c-Cbl TKB domain


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MetRD peptide Chains: A, C
Molecule details ›
Chains: A, C
Length: 11 amino acids
Theoretical weight: 1.38 KDa
Source organism: Synthetic construct
Expression system: Not provided
E3 ubiquitin-protein ligase CBL Chains: B, D
Molecule details ›
Chains: B, D
Length: 329 amino acids
Theoretical weight: 38.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P22681 (Residues: 25-351; Coverage: 36%)
Gene names: CBL, CBL2, RNF55
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 52.743Å b: 104.483Å c: 60.548Å
α: 90° β: 90.19° γ: 90°
R R work R free
0.16 0.159 0.18
Expression systems:
  • Not provided
  • Escherichia coli