PDBe 3p95

X-ray diffraction
1.3Å resolution

Human mesotrypsin complexed with bovine pancreatic trypsin inhibitor variant (BPTI-K15R/R17D)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Pancreatic trypsin inhibitor Chain: E
Molecule details ›
Chain: E
Length: 58 amino acids
Theoretical weight: 6.51 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 43.915Å b: 39.095Å c: 68.46Å
α: 90° β: 100.13° γ: 90°
R-values:
R R work R free
0.111 0.11 0.132
Expression systems:
  • Escherichia coli
  • Komagataella pastoris