PDBe 3p71

X-ray diffraction
2.7Å resolution

Crystal structure of the complex of LCMT-1 and PP2A


Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine = S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leucine carboxyl methyltransferase 1 Chain: T
Molecule details ›
Chain: T
Length: 334 amino acids
Theoretical weight: 38.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9UIC8 (Residues: 1-334; Coverage: 100%)
  • Best match: Q9UIC8-2 (Residues: 60-357)
Gene names: CGI-68, LCMT, LCMT1
Sequence domains: Leucine carboxyl methyltransferase
Structure domains: Vaccinia Virus protein VP39
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 304 amino acids
Theoretical weight: 35.04 KDa
Source organism: Homo sapiens
Expression system: Heliothis
  • Canonical: P67775 (Residues: 1-309; Coverage: 98%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 48.802Å b: 65.305Å c: 193.092Å
α: 90° β: 90° γ: 90°
R R work R free
0.195 0.192 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Heliothis