PDBe 3p37

X-ray diffraction
2.38Å resolution

Polo-like kinase I Polo-box domain in complex with FDPPLHSpTA phosphopeptide from PBIP1


Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
phosphopeptide Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 11 amino acids
Theoretical weight: 1.09 KDa
Source organism: Synthetic construct
Expression system: Not provided
Serine/threonine-protein kinase PLK1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 232 amino acids
Theoretical weight: 26.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P53350 (Residues: 371-594; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: Polo-box like domain

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 58.969Å b: 88.658Å c: 67.59Å
α: 90° β: 113.48° γ: 90°
R R work R free
0.235 0.232 0.288
Expression systems:
  • Not provided
  • Escherichia coli