› 3ou2

X-ray diffraction
1.5Å resolution

DhpI-SAH complex structure

Released:
DOI: 10.2210/pdb3ou2/pdb
PDB entry 3ou2 coloured by chain, front view.
PDB entry 3ou2 coloured by chain, side view.
PDB entry 3ou2 coloured by chain, top view.
Source organism: Streptomyces luridus
Primary publication:
Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis.
Lee JH, Bae B, Kuemin M, Circello BT, Metcalf WW, Nair SK, van der Donk WA
Proc Natl Acad Sci U S A 107 17557-62 (2010)
PMID: 20876132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 24.61 KDa
Source organism: Streptomyces luridus
Expression system: Escherichia coli
UniProt:
  • Canonical: D7PC21 (Residues: 1-218; Coverage: 100%)
Gene name: dhpI
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 75.519Å b: 75.519Å c: 70.657Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.221
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
1 more

3 mentions without citation

Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis.
Lee et al. (2010)
2 more