› 3orc

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF AN ENGINEERED CRO MONOMER BOUND NONSPECIFICALLY TO DNA

Released:
DOI: 10.2210/pdb3orc/pdb
PDB entry 3orc coloured by chain, front view.
PDB entry 3orc coloured by chain, side view.
PDB entry 3orc coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Crystal structure of an engineered Cro monomer bound nonspecifically to DNA: possible implications for nonspecific binding by the wild-type protein.
Albright RA, Mossing MC, Matthews BW
Protein Sci 7 1485-94 (1998)
PMID: 9684880

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116666 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.24 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 2-56, 57-61; Coverage: 91%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor
DNA (5'-D(*TP*AP*TP*CP*GP*AP*TP*A)-3') Chains: R, S
Molecule details ›
Chains: R, S
Length: 8 nucleotides
Theoretical weight: 2.43 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 45.72Å b: 60.66Å c: 45.72Å
α: 90° β: 112.7° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A structural basis for processivity.
Breyer et al. (2001)
1 more

2 mentions without citation

Insights into protein-DNA interactions through structure network analysis.
Sathyapriya et al. (2008)
1 more