PDBe 3orc

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF AN ENGINEERED CRO MONOMER BOUND NONSPECIFICALLY TO DNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.24 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 2-56, 57-61; Coverage: 91%)
Gene names: cro, lambdap57
Structure domains: CRO Repressor
DNA (5'-D(*TP*AP*TP*CP*GP*AP*TP*A)-3') Chains: R, S
Molecule details ›
Chains: R, S
Length: 8 nucleotides
Theoretical weight: 2.43 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 45.72Å b: 60.66Å c: 45.72Å
α: 90° β: 112.7° γ: 90°
Expression system: Escherichia coli