› 3oer

X-ray diffraction
3.2Å resolution

Crystal structure of trimeric frataxin from the yeast saccharomyces cerevisiae, complexed with cobalt

Released:
DOI: 10.2210/pdb3oer/pdb
PDB entry 3oer coloured by chain, front view.
PDB entry 3oer coloured by chain, side view.
PDB entry 3oer coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Oligomerization propensity and flexibility of yeast frataxin studied by X-ray crystallography and small-angle X-ray scattering.
Söderberg CA, Shkumatov AV, Rajan S, Gakh O, Svergun DI, Isaya G, Al-Karadaghi S
J Mol Biol 414 783-97 (2011)
PMID: 22051511

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Frataxin homolog, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07540 (Residues: 52-174; Coverage: 71%)
Gene names: YDL120W, YFH1
Sequence domains: Frataxin-like domain
Structure domains: Frataxin/CyaY

Ligands and Environments

1 bound ligand:
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: I213
Unit cell:
a: 121.2Å b: 121.2Å c: 121.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.204 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Frataxin: a protein in search for a function.
Pastore et al. (2013)
1 more

4 mentions without citation

Tangled web of interactions among proteins involved in iron-sulfur cluster assembly as unraveled by NMR, SAXS, chemical crosslinking, and functional studies.
Kim et al. (2015)
3 more