PDBe 3o2s

X-ray diffraction
2.5Å resolution

Crystal structure of the human symplekin-Ssu72 complex

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Symplekin Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92797 (Residues: 30-360; Coverage: 26%)
Gene names: SPK, SYMPK
Sequence domains: Domain of unknown function (DUF3453)
Structure domains: Leucine-rich Repeat Variant
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP77 (Residues: 1-194; Coverage: 100%)
Gene names: HSPC182, PNAS-120, SSU72
Sequence domains: Ssu72-like protein
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21212
Unit cell:
a: 99.396Å b: 113.564Å c: 59.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.266
Expression system: Escherichia coli