PDBe 3nmz

X-ray diffraction
3.01Å resolution

Crystal structure of APC complexed with Asef

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adenomatous polyposis coli protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 50.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25054 (Residues: 303-739; Coverage: 15%)
Gene names: APC, DP2.5
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Rho guanine nucleotide exchange factor 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 116 amino acids
Theoretical weight: 12.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR80 (Residues: 170-276; Coverage: 16%)
Gene names: ARHGEF4, KIAA1112
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: R3
Unit cell:
a: 163.175Å b: 163.175Å c: 242.593Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.249 0.284
Expression system: Escherichia coli