PDBe 3nmx

X-ray diffraction
2.3Å resolution

Crystal structure of APC complexed with Asef

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adenomatous polyposis coli protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 354 amino acids
Theoretical weight: 39.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25054 (Residues: 407-751; Coverage: 12%)
Gene names: APC, DP2.5
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Rho guanine nucleotide exchange factor 4 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 25 amino acids
Theoretical weight: 2.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NR80 (Residues: 170-194; Coverage: 4%)
Gene names: ARHGEF4, KIAA1112

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 147.79Å b: 92.18Å c: 107.84Å
α: 90° β: 93.77° γ: 90°
R-values:
R R work R free
0.21 0.208 0.254
Expression systems:
  • Escherichia coli
  • Not provided