PDBe 3n85

X-ray diffraction
3.2Å resolution

Crystallographic trimer of HER2 extracellular regions in complex with tryptophan-rich antibody fragment

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab37 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Structure domains: Immunoglobulins
Fab37 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 68.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-646; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1, ALS BEAMLINE 5.0.2
Spacegroup: P6322
Unit cell:
a: 182.22Å b: 182.22Å c: 330.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.23
Expression systems:
  • Escherichia coli
  • Cricetulus griseus