PDBe 3n5p

X-ray diffraction
2.39Å resolution

Structure of endothelial nitric oxide synthase heme domain complexed with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine

Released:
Source organism: Bos taurus

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, endothelial Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 49.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29473 (Residues: 39-482; Coverage: 37%)
Gene name: NOS3
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 60.044Å b: 106.099Å c: 158.602Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.26
Expression system: Escherichia coli