PDBe 3n3a

X-ray diffraction
1.99Å resolution

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with Reduced NrdI

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein NrdI Chains: C, D
Molecule details ›
Chains: C, D
Length: 153 amino acids
Theoretical weight: 17.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A772 (Residues: 1-133; Coverage: 98%)
Gene names: JW2649, b2674, nrdI, ygaO
Sequence domains: NrdI Flavodoxin like
Structure domains: Rossmann fold
Ribonucleoside-diphosphate reductase 2 subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 36.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37146 (Residues: 1-319; Coverage: 100%)
Gene names: JW2651, b2676, nrdF, ygaD
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 74.863Å b: 90.678Å c: 143.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.227
Expression system: Escherichia coli