PDBe 3n37

X-ray diffraction
1.65Å resolution

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Reaction catalysed:
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin. 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase 2 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 36.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37146 (Residues: 1-319; Coverage: 100%)
Gene names: JW2651, b2676, nrdF, ygaD
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6522
Unit cell:
a: 78.142Å b: 78.142Å c: 266.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.185
Expression system: Escherichia coli