PDBe 3n06

X-ray diffraction
2Å resolution

A mutant human Prolactin receptor antagonist H27A in complex with the extracellular domain of the human prolactin receptor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Prolactin Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01236 (Residues: 43-227; Coverage: 93%)
Gene name: PRL
Structure domains: Growth Hormone; Chain: A;
Prolactin receptor Chain: B
Molecule details ›
Chain: B
Length: 210 amino acids
Theoretical weight: 24.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16471 (Residues: 25-234; Coverage: 35%)
  • Best match: P16471-8 (Residues: 1-163)
Gene name: PRLR
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P65
Unit cell:
a: 123.419Å b: 123.419Å c: 72.665Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.241
Expression system: Escherichia coli BL21