› 3mwm

X-ray diffraction
2.4Å resolution

Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur

Released:
DOI: 10.2210/pdb3mwm/pdb
PDB entry 3mwm coloured by chain, front view.
PDB entry 3mwm coloured by chain, side view.
PDB entry 3mwm coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur.
Shin JH, Jung HJ, An YJ, Cho YB, Cha SS, Roe JH
Proc Natl Acad Sci U S A 108 5045-50 (2011)
PMID: 21383173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metal uptake regulation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 14.7 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L2H5 (Residues: 2-139; Coverage: 99%)
Gene names: SCC121.11, SCO2508
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 103.599Å b: 36.978Å c: 71.645Å
α: 90° β: 103.26° γ: 90°
R-values:
R R work R free
0.206 0.206 0.246
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
16 more

18 mentions without citation

Specificity of metal sensing: iron and manganese homeostasis in Bacillus subtilis.
Helmann JD. (2014)
17 more