PDBe 3mtn

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 21 Chains: A, C
Molecule details ›
Chains: A, C
Length: 373 amino acids
Theoretical weight: 41.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UK80 (Residues: 209-562; Coverage: 63%)
Gene names: PP1490, USP21, USP23
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
Structure domains: Cysteine proteinases
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 85 amino acids
Theoretical weight: 9.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG48 (Residues: 533-608; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 169.065Å b: 58.235Å c: 133.596Å
α: 90° β: 121.19° γ: 90°
R-values:
R R work R free
0.22 0.218 0.273
Expression system: Escherichia coli BL21