PDBe 3mgt

X-ray diffraction
2.2Å resolution

Crystal structure of a H5-specific CTL epitope variant derived from H5N1 influenza virus in complex with HLA-A*0201

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Hemagglutinin Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 10 amino acids
Theoretical weight: 1.24 KDa
Source organism: Influenza A virus (A/Anhui/1/2005(H5N1))
Expression system: Not provided
UniProt:
  • Canonical: Q1WDM0 (Residues: 205-214; Coverage: 2%)
Gene name: HA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 63.172Å b: 79.304Å c: 86.739Å
α: 90.02° β: 89.99° γ: 89.96°
R-values:
R R work R free
0.194 0.192 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided