PDBe 3mca

X-ray diffraction
2.74Å resolution

Structure of the Dom34-Hbs1 Complex and implications for its role in No-Go decay

Released:
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure of the Dom34-Hbs1 complex and implications for no-go decay.
Nat. Struct. Mol. Biol. 17 1233-40 (2010)
PMID: 20890290

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein dom34 Chain: B
Molecule details ›
Chain: B
Length: 390 amino acids
Theoretical weight: 44.4 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9USL5 (Residues: 1-390; Coverage: 100%)
Gene names: SPCC18B5.06, dom34
Sequence domains:
Structure domains:
Elongation factor 1 alpha-like protein Chain: A
Molecule details ›
Chain: A
Length: 592 amino acids
Theoretical weight: 66.1 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74774 (Residues: 1-592; Coverage: 100%)
Gene names: SPBC25B2.01, SPBC2G5.08, hbs1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 76.741Å b: 113.423Å c: 124.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.247 0.286
Expression system: Escherichia coli