PDBe 3m0d

X-ray diffraction
2.8Å resolution

Crystal structure of the TRAF1:TRAF2:cIAP2 complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TNF receptor-associated factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12933 (Residues: 266-329; Coverage: 13%)
Gene names: TRAF2, TRAP3
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
TNF receptor-associated factor 1 Chain: C
Molecule details ›
Chain: C
Length: 65 amino acids
Theoretical weight: 7.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13077 (Residues: 181-244; Coverage: 15%)
Gene names: EBI6, TRAF1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Baculoviral IAP repeat-containing protein 3 Chain: D
Molecule details ›
Chain: D
Length: 75 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13489 (Residues: 26-99; Coverage: 12%)
Gene names: API2, BIRC3, MIHC, RNF49
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I41
Unit cell:
a: 91.867Å b: 91.867Å c: 88.702Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.197 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli