PDBe 3lu9

X-ray diffraction
1.8Å resolution

Crystal structure of human thrombin mutant S195A in complex with the extracellular fragment of human PAR1

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of thrombin bound to the uncleaved extracellular fragment of PAR1.
J. Biol. Chem. 285 15393-8 (2010)
PMID: 20236938

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 46 amino acids
Theoretical weight: 5.37 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 318-363; Coverage: 8%)
Gene name: F2
Structure domains: Thrombin light chain domain
Thrombin heavy chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 259 amino acids
Theoretical weight: 29.76 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Proteinase-activated receptor 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 25 amino acids
Theoretical weight: 3 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P25116 (Residues: 33-57; Coverage: 6%)
Gene names: CF2R, F2R, PAR1, TR

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P1
Unit cell:
a: 46.334Å b: 50.171Å c: 85.502Å
α: 76.39° β: 83.93° γ: 73.74°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Cricetulus griseus