› 3lad

X-ray diffraction
2.2Å resolution

REFINED CRYSTAL STRUCTURE OF LIPOAMIDE DEHYDROGENASE FROM AZOTOBACTER VINELANDII AT 2.2 ANGSTROMS RESOLUTION. A COMPARISON WITH THE STRUCTURE OF GLUTATHIONE REDUCTASE

Released:
DOI: 10.2210/pdb3lad/pdb
PDB entry 3lad coloured by chain, front view.
PDB entry 3lad coloured by chain, side view.
PDB entry 3lad coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Refined crystal structure of lipoamide dehydrogenase from Azotobacter vinelandii at 2.2 A resolution. A comparison with the structure of glutathione reductase.
Mattevi A, Schierbeek AJ, Hol WG
J Mol Biol 220 975-94 (1991)
PMID: 1880807

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 49.49 KDa
Source organism: Azotobacter vinelandii
Expression system: Not provided
UniProt:
  • Canonical: P18925 (Residues: 2-477; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.13Å b: 83.86Å c: 191.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 not available not available
Expression system: Not provided

Quick links

Citations

7 review citations

Thioredoxin reductase two modes of catalysis have evolved.
Williams et al. (2000)
6 more

6 mentions without citation

Toward a resolution of the introns early/late debate: only phase zero introns are correlated with the structure of ancient proteins.
de Souza et al. (1998)
5 more