PDBe 3l33

X-ray diffraction
2.48Å resolution

Human mesotrypsin complexed with amyloid precursor protein inhibitor(APPI)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Soluble APP-beta Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 52 amino acids
Theoretical weight: 5.82 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P05067 (Residues: 290-341; Coverage: 7%)
Gene names: A4, AD1, APP

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Unit cell:
a: 92.793Å b: 130.067Å c: 132.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.256
Expression systems:
  • Escherichia coli
  • Komagataella pastoris