PDBe 3l2c

X-ray diffraction
1.87Å resolution

Crystal Structure of the DNA Binding Domain of FOXO4 Bound to DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O4 Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P98177 (Residues: 86-187; Coverage: 20%)
Gene names: AFX, AFX1, FOXO4, MLLT7
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
FOXO consensus binding sequence, plus strand Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
FOXO consensus binding sequence, minus strand Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2221
Unit cell:
a: 40.81Å b: 71.7Å c: 131.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided