PDBe 3l2b

X-ray diffraction
2.27Å resolution

Crystal structure of the CBS and DRTGG domains of the regulatory region of Clostridium perfringens pyrophosphatase complexed with activator, diadenosine tetraphosphate

Released:

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalt-dependent inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.97 KDa
Source organism: Clostridium perfringens str. 13
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8XIQ9 (Residues: 66-306; Coverage: 44%)
Gene name: CPE2055
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 63.847Å b: 71.764Å c: 116.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.259
Expression system: Escherichia coli