PDBe 3kuc

X-ray diffraction
1.92Å resolution

Complex of Rap1A(E30D/K31E)GDP with RafRBD(A85K/N71R)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rap-1A Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62834 (Residues: 1-167; Coverage: 91%)
Gene names: KREV1, RAP1A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
RAF proto-oncogene serine/threonine-protein kinase Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04049 (Residues: 51-131; Coverage: 13%)
Gene names: RAF, RAF1
Sequence domains: Raf-like Ras-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 43.12Å b: 68.54Å c: 101.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.209
Expression system: Escherichia coli