PDBe 3ku4

X-ray diffraction
2.1Å resolution

Trapping of an oxocarbenium ion intermediate in UP crystals

Released:
Source organism: Bos taurus

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 33.92 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5PJH9 (Residues: 1-309; Coverage: 100%)
Gene name: UPP1
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41
Unit cell:
a: 74.108Å b: 74.108Å c: 266.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.225
Expression system: Escherichia coli BL21(DE3)