PDBe 3kqi

X-ray diffraction
1.78Å resolution

crystal structure of PHF2 PHD domain complexed with H3K4Me3 peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
H3K4Me3 peptide Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
Lysine-specific demethylase PHF2 Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75151 (Residues: 1-70; Coverage: 6%)
Gene names: CENP-35, KIAA0662, PHF2
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 77.757Å b: 77.758Å c: 72.116Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.218
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)