PDBe 3kmt

X-ray diffraction
1.78Å resolution

Crystal structure of vSET/SAH/H3 ternary complex

Released:
Primary publication:
Dimerization of a viral SET protein endows its function.
Proc. Natl. Acad. Sci. U.S.A. 107 18433-8 (2010)
PMID: 20937900

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone H3K27 methylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 119 amino acids
Theoretical weight: 13.61 KDa
Source organism: Paramecium bursaria Chlorella virus 1
UniProt:
  • Canonical: NEW O41094 (Residues: 1-119; Coverage: 100%)
Gene name: A612L
Sequence domains: SET domain
Structure domains: SET domain
Histone H3.1 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 8 amino acids
Theoretical weight: 816 Da
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P68431 (Residues: 26-33; Coverage: 6%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 155.085Å b: 38.722Å c: 73.208Å
α: 90° β: 107.8° γ: 90°
R-values:
R R work R free
0.21 0.196 0.224