PDBe 3jz1

X-ray diffraction
1.6Å resolution

Crystal structure of human thrombin mutant N143P in E:Na+ form

Released:
Source organism: Homo sapiens
Primary publication:
Mutant N143P reveals how Na+ activates thrombin.
J. Biol. Chem. 284 36175-85 (2009)
PMID: 19846563

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 29.76 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 119.979Å b: 47.67Å c: 50.688Å
α: 90° β: 92.29° γ: 90°
R-values:
R R work R free
0.203 0.203 0.216
Expression system: Cricetulus griseus