PDBe 3jt0

X-ray diffraction
2.39Å resolution

Crystal Structure of the C-terminal fragment (426-558) Lamin-B1 from Homo sapiens, Northeast Structural Genomics Consortium Target HR5546A

Released:
Source organism: Homo sapiens
Entry authors: Kuzin A, Abashidze M, Seetharaman J, Sahdev S, Xiao R, Ciccosanti C, Belote RL, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lamin-B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20700 (Residues: 426-558; Coverage: 23%)
Gene names: LMN2, LMNB, LMNB1
Sequence domains: Lamin Tail Domain
Structure domains: Lamin Tail domain

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 39.437Å b: 72.793Å c: 81.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.245
Expression system: Escherichia coli BL21(DE3)