PDBe 3jsv

X-ray diffraction
2.7Å resolution

Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 77 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
NF-kappa-B essential modulator Chains: C, D
Molecule details ›
Chains: C, D
Length: 94 amino acids
Theoretical weight: 11.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O88522 (Residues: 250-343; Coverage: 23%)
Gene names: Ikbkg, Nemo
Structure domains: Nemo cc2-lz domain - 1d5 darpin complex

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 55.38Å b: 45.87Å c: 83.29Å
α: 90° β: 99.83° γ: 90°
R-values:
R R work R free
0.25 0.25 0.294
Expression system: Escherichia coli