PDBe 3jqo

X-ray diffraction
2.6Å resolution

Crystal structure of the outer membrane complex of a type IV secretion system

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TraF protein Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n
Molecule details ›
Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n
Length: 227 amino acids
Theoretical weight: 24.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46705 (Residues: 160-386; Coverage: 59%)
Gene name: traF
Sequence domains: Bacterial conjugation TrbI-like protein
TraN protein Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Molecule details ›
Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Length: 34 amino acids
Theoretical weight: 3.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46702 (Residues: 15-48; Coverage: 71%)
Gene name: traN
TraO protein Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o
Molecule details ›
Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o
Length: 135 amino acids
Theoretical weight: 15.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46704 (Residues: 160-294; Coverage: 50%)
Gene name: traO

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 202.4Å b: 211.63Å c: 203.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.259
Expression system: Escherichia coli