PDBe 3jbk

Electron Microscopy
8.2Å resolution

Cryo-EM reconstruction of the metavinculin-actin interface

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Vinculin Chain: M
Molecule details ›
Chain: M
Length: 273 amino acids
Theoretical weight: 29.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18206 (Residues: 858-1129; Coverage: 24%)
Gene name: VCL

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.2Å
Relevant EMDB volumes: EMD-6447
Expression system: Escherichia coli BL21(DE3)