PDBe 3iqj

X-ray diffraction
1.15Å resolution

Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (10mer)

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
RAF proto-oncogene serine/threonine-protein kinase Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P04049 (Residues: 255-264; Coverage: 2%)
Gene names: RAF, RAF1

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 82.61Å b: 112.56Å c: 62.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.127 0.153
Expression system: Not provided