PDBe 3ik5

X-ray diffraction
2.05Å resolution

SIVmac239 Nef in complex with TCR zeta ITAM 1 polypeptide (A63-R80)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Nef Chains: A, C
Molecule details ›
Chains: A, C
Length: 143 amino acids
Theoretical weight: 16.7 KDa
Source organism: Simian immunodeficiency virus - cpz
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31818 (Residues: 95-235; Coverage: 54%)
Gene name: nef
Structure domains: Nef Regulatory Factor
T-cell surface glycoprotein CD3 zeta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 18 amino acids
Theoretical weight: 2.17 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P20963 (Residues: 63-80; Coverage: 13%)
Gene names: CD247, CD3Z, T3Z, TCRZ

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 47.417Å b: 47.421Å c: 183.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.184
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided