PDBe 3i6k

X-ray diffraction
2.8Å resolution

Newly identified epitope from SARS-CoV membrane protein complexed with HLA-A*0201

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Membrane protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 10 amino acids
Theoretical weight: 1.01 KDa
Source organism: SARS coronavirus TJF
Expression system: Not provided
UniProt:
  • Canonical: Q692E0 (Residues: 60-69; Coverage: 5%)
Gene name: M

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 65.54Å b: 108.028Å c: 134.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided