PDBe 3hyf

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase/RNaseH; Ribonuclease HI Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.92 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q72547 (Residues: 427-506, 518-561; Coverage: 22%)
  • Canonical: P0A7Y4 (Residues: 79-103; Coverage: 16%)
  • nullnull
Gene names: JW0204, b0214, dasF, herA, pol, rnh, rnhA, sdrA
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I212121
Unit cell:
a: 39.717Å b: 90.326Å c: 113.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.232
Expression system: Escherichia coli