PDBe 3hr4

X-ray diffraction
2.5Å resolution

Human iNOS Reductase and Calmodulin Complex

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitric oxide synthase, inducible Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 219 amino acids
Theoretical weight: 25.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35228 (Residues: 503-715; Coverage: 19%)
Gene names: NOS2, NOS2A
Sequence domains: Flavodoxin
Structure domains: Rossmann fold
Calmodulin-2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP24 (Residues: 1-149; Coverage: 100%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 36.6Å b: 160.84Å c: 127.77Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.237 0.237 0.309
Expression system: Escherichia coli