PDBe 3hmx

X-ray diffraction
3Å resolution

Crystal structure of ustekinumab FAB/IL-12 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
USTEKINUMAB FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Structure domains: Immunoglobulins
USTEKINUMAB FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Structure domains: Immunoglobulins
Interleukin-12 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 197 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29459 (Residues: 23-219; Coverage: 100%)
Gene names: IL12A, NKSF1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 55.092Å b: 116.435Å c: 182.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.211 0.303
Expression system: Homo sapiens