PDBe 3hg1

X-ray diffraction
3Å resolution

Germline-governed recognition of a cancer epitope by an immunodominant human T cell receptor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
MHC class I antigen Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WLS4 (Residues: 25-300; Coverage: 94%)
Gene name: HLA-A
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma antigen recognized by T-cells 1 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 26-35; Coverage: 9%)
Gene names: MART1, MLANA
T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 194 amino acids
Theoretical weight: 21.37 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01848 (Residues: 1-18, 19-85; Coverage: 61%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P43
Unit cell:
a: 120.898Å b: 120.898Å c: 81.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.304
Expression systems:
  • Escherichia coli
  • Not provided