PDBe 3hfg

X-ray diffraction
2.3Å resolution

Crystal Structure of Human 11-beta-hydroxysteroid-dehydrogenase Bound to an Sulfonyl-piperazine Inhibitor

Released:

Function and Biology Details

Reaction catalysed:
An 11-beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corticosteroid 11-beta-dehydrogenase isozyme 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28845 (Residues: 24-292; Coverage: 92%)
Gene names: HSD11, HSD11B1, HSD11L, SDR26C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Rigaku FR-E
Spacegroup: P21
Unit cell:
a: 56.438Å b: 152.67Å c: 74.203Å
α: 90° β: 92.41° γ: 90°
R-values:
R R work R free
0.234 0.231 0.285
Expression system: Escherichia coli