PDBe 3h9e

X-ray diffraction
1.72Å resolution

Crystal structure of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) complex with NAD and phosphate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific Chains: O, P
Molecule details ›
Chains: O, P
Length: 346 amino acids
Theoretical weight: 37.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14556 (Residues: 69-407; Coverage: 83%)
Gene names: GAPD2, GAPDH2, GAPDHS, GAPDS, HSD-35, HSD35
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 141.96Å b: 71.78Å c: 81.26Å
α: 90° β: 122.9° γ: 90°
R-values:
R R work R free
0.143 0.143 0.176
Expression system: Escherichia coli