PDBe 3h7h

X-ray diffraction
1.55Å resolution

Crystal structure of the human transcription elongation factor DSIF, hSpt4/hSpt5 (176-273)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription elongation factor SPT4 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63272 (Residues: 2-117; Coverage: 99%)
Gene names: SPT4H, SUPT4H, SUPT4H1
Sequence domains: Spt4/RpoE2 zinc finger
Transcription elongation factor SPT5 Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00267 (Residues: 176-273; Coverage: 9%)
Gene names: SPT5, SPT5H, SUPT5H
Sequence domains: Early transcription elongation factor of RNA pol II, NGN section
Structure domains: N-utilization substance G protein NusG, N-terminal domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 41.096Å b: 52.091Å c: 97.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.222
Expression system: Escherichia coli