PDBe 3h1p

X-ray diffraction
2.61Å resolution

Mature Caspase-7 I213A with DEVD-CHO inhibitor bound to active site

Released:
Source organism: Homo sapiens
Primary publication:
L2' loop is critical for caspase-7 active site formation.
Protein Sci. 18 1459-68 (2009)
PMID: 19530232

Function and Biology Details

Reaction catalysed:
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-7 subunit p20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55210 (Residues: 50-303; Coverage: 84%)
Gene names: CASP7, MCH3
Sequence domains: Caspase domain
Structure domains: Rossmann fold
N-ACETYL-L-ALPHA-ASPARTYL-L-ALPHA-GLUTAMYL-N-[(2S)-1-CARBOXY-3-HYDROXYPROPAN-2-YL]-L-VALINAMIDE Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 488 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P3221
Unit cell:
a: 89.347Å b: 89.347Å c: 186.101Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided