PDBe 3gut

X-ray diffraction
3.59Å resolution

Crystal structure of a higher-order complex of p50:RelA bound to the HIV-1 LTR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor p65 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 273 amino acids
Theoretical weight: 30.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q04206 (Residues: 20-291; Coverage: 49%)
Gene names: NFKB3, RELA
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Nuclear factor NF-kappa-B p50 subunit Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 312 amino acids
Theoretical weight: 34.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19838 (Residues: 41-352; Coverage: 32%)
  • Best match: P19838-3 (Residues: 10-172)
Gene name: NFKB1
Sequence domains: Rel homology DNA-binding domain
Structure domains:
HIV-LTR Core Forward Strand Chains: I, X
Molecule details ›
Chains: I, X
Length: 26 nucleotides
Theoretical weight: 8 KDa
HIV-LTR Core Reverse Strand Chains: J, Y
Molecule details ›
Chains: J, Y
Length: 26 nucleotides
Theoretical weight: 7.98 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P42
Unit cell:
a: 167.493Å b: 167.493Å c: 172.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.301