PDBe 3gq3

X-ray diffraction
1.83Å resolution

MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC5-loop deletion complex

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.87 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84131 (Residues: 2-274; Coverage: 94%)
Gene names: fpg, mutM
Sequence domains: Zinc finger found in FPG and IleRS
Structure domains:
DNA (5'-D(*AP*GP*GP*TP*AP*GP*AP*CP*CP*CP*GP*GP*AP*CP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*GP*TP*CP*CP*GP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 45.085Å b: 92.411Å c: 105.836Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.209
Expression systems:
  • Escherichia coli
  • Not provided