› 3gp6

X-ray diffraction
1.4Å resolution

Crystal structure of PagP in SDS/MPD

Released:
DOI: 10.2210/pdb3gp6/pdb
PDB entry 3gp6 coloured by chain, front view.
PDB entry 3gp6 coloured by chain, side view.
PDB entry 3gp6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler.
Cuesta-Seijo JA, Neale C, Khan MA, Moktar J, Tran CD, Bishop RE, Pomès R, Privé GG
Structure 18 1210-9 (2010)
PMID: 20826347

Function and Biology Details

Reaction catalysed: 
1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine + hexa-acyl lipid A = 2-acyl-sn-glycero-3-phosphocholine + hepta-acyl lipid A
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid A palmitoyltransferase PagP Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 19.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37001 (Residues: 26-186; Coverage: 100%)
Gene names: JW0617, b0622, crcA, pagP, ybeG
Sequence domains: Antimicrobial peptide resistance and lipid A acylation protein PagP
Structure domains: Porin

Ligands and Environments

5 bound ligands:
Ligand SDS 6 x SDS
Ligand MPD 9 x MPD
Ligand MRD 1 x MRD
Ligand SO4 6 x SO4
Ligand LI 2 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6222
Unit cell:
a: 113.228Å b: 113.228Å c: 55.064Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.208
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies.
Chipot et al. (2018)
5 more

19 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
18 more