PDBe 3gcm

X-ray diffraction
2.5Å resolution

Crystal Structure of E. coli polynucleotide phosphorylase bound to RNA and RNase E


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease E Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 41 amino acids
Theoretical weight: 4.34 KDa
Source organism: Escherichia coli
Expression system: Not provided
  • Canonical: A7ZKI9 (Residues: 1021-1061; Coverage: 4%)
Gene names: EcE24377A_1207, rne
Sequence domains: Polyribonucleotide phosphorylase C terminal
Polyribonucleotide nucleotidyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 549 amino acids
Theoretical weight: 59.66 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
  • Canonical: A7ZS61 (Residues: 1-549; Coverage: 77%)
Gene names: EcE24377A_3647, pnp
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 176.335Å b: 176.335Å c: 189.628Å
α: 90° β: 90° γ: 90°
R R work R free
0.201 0.166 0.221
Expression system: Not provided