› 3fxq

X-ray diffraction
1.85Å resolution

Crystal structure of the LysR-type transcriptional regulator TsaR

Released:
DOI: 10.2210/pdb3fxq/pdb
PDB entry 3fxq coloured by chain, front view.
PDB entry 3fxq coloured by chain, side view.
PDB entry 3fxq coloured by chain, top view.
Source organism: Comamonas testosteroni
Primary publication:
Structural studies on the full-length LysR-type regulator TsaR from Comamonas testosteroni T-2 reveal a novel open conformation of the tetrameric LTTR fold.
Monferrer D, Tralau T, Kertesz MA, Dix I, Solà M, Usón I
Mol Microbiol 75 1199-214 (2010)
PMID: 20059681

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator TsaR Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.64 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli
UniProt:
  • Canonical: P94678 (Residues: 1-298; Coverage: 100%)
Gene name: tsaR
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BME 4 x BME
Ligand GOL 1 x GOL
Ligand FMT 2 x FMT
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2
Unit cell:
a: 136.496Å b: 51.489Å c: 107.754Å
α: 90° β: 110.35° γ: 90°
R-values:
R R work R free
0.182 0.18 0.213
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
3 more

5 mentions without citation

Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR).
Ilangovan et al. (2013)
4 more